S.F. Taghizadeh; Gh. Davarynejad; J. Asili; S.H. Nemati; Gh.R. Karimi
Abstract
Chemical composition, antioxidant, antimicrobial and cytotoxicity of the essential oil obtained from the leaf of Pistacia vera L. var. Sarakhs was investigated in the present study. Chemical composition of the essential oil was analyzed using Gas chromatography- Mass Spectrometry (GC-MS) method. ...
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Chemical composition, antioxidant, antimicrobial and cytotoxicity of the essential oil obtained from the leaf of Pistacia vera L. var. Sarakhs was investigated in the present study. Chemical composition of the essential oil was analyzed using Gas chromatography- Mass Spectrometry (GC-MS) method. Twenty eight compounds, representing 98.28% of the total oil, were characterized. The oil was predominated by monoterpene hydrocarbons (48.8%), sesquiterpene hydrocarbons (35.68%) and oxygenated monoterpenes (11.5%). The antimicrobial activity of the essential oil was investigated against four bacterial strains and one fungus. The essential oil showed a good activity against Gram-positive bacteria particularly Staphylococcus aureus (minimum inhibitory concentration [MIC] and minimum bactericidal concentration [MBC] = 16 µg/ml) and Bacillus cereus (minimum inhibitory concentration [MIC] and minimum bactericidal concentration [MBC] = 90 µg/ml). However, Gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa) were resistant to the essential oil (minimum inhibitory concentration [MIC] and minimum bactericidal concentration [MBC] = 135 µg/ml). The antioxidant potential of essential oil was examined using DPPH, FRAP and β-carotene/linoleic acid (BCB) assay. The oil was considerably active in the DPPH assay (IC50 = 19.03 ± 0.005 µg/ml). Moreover, in vitro cytotoxic activity was assessed against three cancer cell lines (MCF-7, PC3 and DU-145) using Alamar blue assay, with IC50 value less than 32.20 µg/ml for MCF-7 cells.
M. Nasseri; Sh. Golmohammadzadeh; H. Aroiee; M.R. Jaafari; H. Neamati
Abstract
In the present study, solid lipid nanoparticles (SLNs) were used as carriers of essential oil to overcome the problem of essential oil use (evaporation and degradation of some active components in the presence of air, light, moisture, and high temperatures) and increase the essential oil efficiency for ...
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In the present study, solid lipid nanoparticles (SLNs) were used as carriers of essential oil to overcome the problem of essential oil use (evaporation and degradation of some active components in the presence of air, light, moisture, and high temperatures) and increase the essential oil efficiency for controlling Alternaria solani, Rhizoctonia solani and Rhizopus stolonifer. This experiment was tested in vitro on PDA in Nanotechnology Research Center, School of Pharmacy, University of Medical Sciences of Mashhad and Faculty of Agriculture, Ferdowsi University of Mashhad. The study was conducted in a completely randomized design (CRD) whit three replications. Solid lipid nanoparticles containing essential oil at four concentrations of 20, 50, 100, and 200 ml per liter were applied on the potato dextrose agar medium. SLN containing Zataria multiflora Boiss. essential oil (ZM-SLN) were prepared by high shear homogenization and ultra sound method. The size of SLNs containing essential oil was less than 200 nm, and PdI and ZP were calculated to be 0.483 and -42.6 mv, respectively. The SLNs were spherical in shape. According to the obtained results, the minimum inhibitory concentration of the essential oil for all three fungi was 200 μlL-1. However, the minimum inhibitory concentration of SLN-ZM for Rh. stolonifer and R. solani was 50μlL-1, and for A. solani, it was calculated to be 100μlL-1. Our results clearly showed that SLNs could be suitable carriers for the Zataria multiflora essential oil.
S. Beigi; M. Azizi; S.H. Nemati; V. Rowshan
Abstract
One of the most important limiting factors in production of medicinal plants in arid and semiarid regions of the world is water shortage or drought stress. Basil (Ocimum basilicum L. var. "Keshkeny levelu") is a valuable medicinal plant and sensitive to water stress. Therefore, the current study was ...
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One of the most important limiting factors in production of medicinal plants in arid and semiarid regions of the world is water shortage or drought stress. Basil (Ocimum basilicum L. var. "Keshkeny levelu") is a valuable medicinal plant and sensitive to water stress. Therefore, the current study was aimed to increase water use efficiency in basil production by conducting a factorial experiment based on randomized complete blocks design with three replications. The treatments were two superabsorbent polymers namely Terracottem™ (industrial) and Plantago ovata mucilage (Herbal) at four concentrations (0, 0.1%, 0.2% and 0.3% w/w on the basis of soil weight) with two application methods (mixed with soil only and mixed with soil+roots). The measured traits were morphological: leaf number, leaf area, shoot to root ratio, biological: number of seeds/plant, seed weight/plant, and water use efficiency, as well as two other important characteristics such as essential oil percentage and yield at flowering time. Results showed that the simple effect of treatments and their interaction had significant and positive effects on morphological, biological and biochemical traits, measured in this experiment. The best results in vegetative and generative characteristics were obtained by application of Plantagoovata mucilage and the highest essential oil percentage and yield were recorded by using Terracottem superabsorbent. The best results in both compounds were obtained at concentrations of 0.1% and 0.2% with application method in soil+roots and soil only, respectively. In conclusion, both compounds (terracottem™ and Plantago ovata) were able to reduce the adverse effects of drought stress and improved water use efficiency in basil cultivation.
M. Azizi; F. Rezwanee; M. Hassanzadeh Khayat; A. Lackzian; H. Neamati
Volume 24, Issue 1 , May 2008, , Pages 82-93
Abstract
German chamomile (Matricaria recutita) is one of the most important essential oil bearing plants that its essential oil constituent (Chamazulene, Bisabolol and Bisabolol oxide A, B) is used in different medicinal industries. In this research, effect of different levels of vermicompost and irrigation ...
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German chamomile (Matricaria recutita) is one of the most important essential oil bearing plants that its essential oil constituent (Chamazulene, Bisabolol and Bisabolol oxide A, B) is used in different medicinal industries. In this research, effect of different levels of vermicompost and irrigation was evaluated on morphological characteristics and essential oil content of “Goral” an improved German chamomile. The research was conducted in pot and greenhouse system. The treatments included four amounts of vermicompost (0, 5, 10 and 15% w/w) and three irrigation regimes (2 mm per week, 4mm per 2 weeks and 2 mm per 2 weeks). The studied factors were flower dry yield, plant height, flowering time, anthodia height, anthodia diameter, oil content (%) and essential oil yield. The results indicated that the vermicompost application improved plant height, early flowering, flowers dry weight, anthodia height and diameter significantly. Irrigation also affects plant height, flower dry weight and flowering time significantly and 2mm irrigation per two weeks was the best method and 2mm irrigation per week increased anthodia height and diameter significantly. The interactions between vermicompost and irrigation was significant as plant height (64.82 cm), flowers dry weight(7.84 gr per pot) and flowering time (35.5 days) as concerned and the best treatments was 15% vermicompost and irrigation 2mm per two weeks. The highest essential oil yield detected in 10% vermicompost and irrigation 4mm per two weeks (2.23 mg/pot). According to the results, it appears that 15% vermicompost plus 2 mm irrigation per two weeks was the best treatment to produce the flower yield in Goral cultivar of German chamomile in organic system.
